J. A. Maangi1*, Bargul, J. L.1, Chalo, R. M.2, Korir, R. K.2
1Department of Biochemistry, Faculty of Science, Jomo Kenyatta University of Agriculture & Technology, 62000-00200, Nairobi, Kenya
2Crop Improvement and Management, Tea Research Institute (TRI), 820-20200, Kericho, Kenya
This study was conducted to assess the effectiveness of simple sequence repeat (SSR) markers in separating interspecific hybrids of tea (Camellia sinensis). A total of 20 SSR primers (5 EST-SSR and 15 microsatellite markers) were screened against three genotypes, i.e., 570/2, 688/1, and 83/1 from 2005, 2007, and 1999 trials, respectively. Genomic DNA was isolated using the CTAB method and amplified using specific PCR conditions for SSR primers. Subsequently, amplicons were separated on 2.0% agarose gel. The polymorphism information content (PIC) of 10 primers was ≥0.5 with a mean of 0.60. A total of 52 alleles (1-9 alleles per locus) were detected. Based on PIC (≥0.5) and the number of polymorphic bands (≥ 2), a set of eight primer-pairs (TM 134, TM 179, TM 51, TM 58, A37, A 47, Camsin M3, Camsin M5, and Camjap A2) were determined as ideal for genetic diversity studies in interspecific tea hybrids. These markers can be utilized for unambiguous detection and marker-assisted selection of tea hybrids to exploit their full potential.
Keywords: Interspecific hybrids, microsatellite marker, simple sequence repeat markers, polymorphic, Camellia sinensis